A Point of Reckoning: Part III

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A Point of Reckoning – Part III

by

Clifford E Carnicom
Oct 06 2017

 

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A common set of organic components has been identified within a wide variety of environmental and biological samples. These components are comprised of organic functional groups and structures that are found in each of the following sample types:

 

  1. The “Environmental Filament” material that has been under investigation by Carnicom Institute for a period that now approaches two decades. This is the same material type that was originally sent to the U.S. Environmental Protection Agency in January of the year 2000 with a request for identification on behalf of the public welfare. The Agency refused to perform that investigation or examination.

 

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Unusual airborne “Environmental Filament” material of identical nature sent to the U.S. Environmental Protection Agency in 2000. A request for identification was made at that time. The request was not fulfilled.

 

 

2. An isolated and specific protein that is derived from the microorganism tentatively identified as a ‘cross-domain bacteria (CDB) as described more extensively on this site. This protein is described in greater detail in the paper entitled, Morgellons: Unique Protein Isolated and Characterized (Aug 2017).

 

3. An extraction from a HEPA air filter that has run continuously for approximately one year. Filters that have been subjected to both indoor and outdoor air show similar sample materials to be collected.

indoor_hepa.jpg outdoor_hepa.jpg

Typical HEPA air filter (indoor and outdoor) sample material used for extraction and subsequent infrared analysis of organic composition. These samples are described in more detail in the paper entitled “A Point of Reckoning: Part I”, Aug 2017.

 

4. Organic extractions from concentrated and multiple rainfall samples.

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Concentrated rainfall samples in comparison to distilled water. Contamination of the water is visually apparent. Additional information regarding rainfall analyses is available on this site.

 

5. A set of biological samples, including that of human hair, saliva and blood have been examined via infrared analysis as a portion of this report. Hair samples require chemical digestion and all samples require the complete removal of water from the sample.

 

6. Skin exfoliation samples from an individual that exhibits symptoms characteristic of the Morgellons condition have also been examined via digestion, digestion and infrared techniques.

 

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Observed skin that exhibits symptoms
characteristic of the Morgellons condition.

Filament sample recorded (one of several)
within a portion of the skin condition shown
to the left. Magnification approx. 150x.

 

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Infrared spectra of a variety of environmental and biological samples that share a common set of organic components. The sample types include the “Environmental Filament”, a specific and isolated microorganism protein, a HEPA air filter extract, a concentrated rainfall sample, hair, saliva and blood samples, and a skin exfoliate sample. Although all sample types have been collected and prepared by vastly different methods and are of varying concentrations, a set of organic functional groups is common to each sample.  These occur within the ‘functional group window’ of the infrared spectra shown.

 

The laboratory methods of analysis include, in part, that of:

Organic extraction methods
Liquid column (low pressure) chromatography
Ultraviolet spectroscopy
Visible light spectroscopy (colorimetric test)
Bradford test for protein
Evaporative techniques
Near Infrared Analysis
Infrared Analysis.

A database of more than 6500 infrared spectra (National Institutes of Technology –NIST and collected) has been used to prepare this research paper.

The functional groups within the analyses that are of heightened interest and that appear to share commonality include those of the phenols, organic acids, isothiocyanates, and the amides. There are numerous implications within this set of functional groups and their combined properties that provide a basis for extended research, investigations trials,and the aggregation of resources and funding for the same in the future .

 

Clifford E Carnicom
Oct 06 2017

Born Clifford Bruce Stewart
Jan 19 1953

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Bean Growth Report

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Bean Growth Report

by

Clifford E Carnicom
Oct 03 2017

The growth of beans (Vigna unguiculata) that have been subjected to a specific and isolated protein for two weeks is now complete This protein is described in greater detail in the paper entitled, Morgellons: Unique Protein Isolated and Characterized (Aug 2017). This protein is derived from the microorganism tentatively identified as a ‘cross-domain bacteria (CDB) as described more extensively on this site.

The protein concentration solution applied to the seeds is 2% by weight. Control solutions with the use of water alone are conducted in parallel for comparison.

The result of this experiment is that germination and growth from the beans is essentially terminated by the presence of this protein at this concentration level. The control seeds have germinated and flourished normally. Additional trials with a lower concentration of the protein in solution are planned.

Photographs that demonstrate the condition of growth in both cases are shown below:

 

bean_control.jpg

The growth of beans (Black eyed pea) under control conditions of water nutrient solution alone is recorded above.  Growth appears to be entirely normal and healthy over the two week period. A bean that remained under the water level in the control solution is trapped by the root of the plant to the right.

 

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The halted and damaged growth of the same bean species after being subjected to the isolated and specific protein under study. The origin and nature of this protein have been described within the research on this site. The concentration of the protein solution is 2% by weight. The time period for growth is two weeks.  The growth process has been terminated and it shows significant harm to the plant; in addition, the solution has fostered a fungal attack upon the seeds. A highly stunted from of germination occurs at the lower right of the seed shown to the left; there is no germination of the seed shown to the right. The vast majority of the beans subjected to the protein show no visible germination.

 

This report demonstrates that the agricultural, biological and health impacts from this particular protein are likely to be significant and detrimental. Additional tests reported and underway support this finding.

 

Clifford E Carnicom
Oct 03 2017

Born Clifford Bruce Stewart
Jan 19 1953

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Protozoa Motility and Mortality

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Protozoa Motility and Mortality

by

Clifford E Carnicom
Sep 29 2017

 

A protozoa culture has been subjected to a specific and isolated protein. This protein is described in greater detail in the paper entitled, Morgellons: Unique Protein Isolated and Characterized (Aug 2017). This protein is derived from the microorganism tentatively identified as a ‘cross-domain bacteria (CDB) as described more extensively on this site.

The concentration of the protein concentration that is applied to the protozoa is approximately 0.1% by weight to volume of water; this is a rather weak solution in comparison to other biological trials that are underway. Control solutions with the use of water alone are conducted in parallel for comparison. The protozoa culture is dominated by common species, such as paramecium, euglena, stentor, volvox, and amoeba.

The result of this experiment is that the motility of the protozoa is diminished significantly after a specific time period in comparison to that of the control culture. The mortality rate of the protozoa is also increased in a corresponding fashion in comparison to that of the control and the rate of the mortality appears to be in direct proportion to the size and mass of the species. The control protozoa have not demonstrated any harm or degradation during an extended observation period.

Time lapse images which demonstrate some of the observed changes in the viability of the culture are shown below.

 

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Time lapse images of protozoa cultured in control water infusion nutrient solution. These images were captured after the extended time interval of approximately 3 hours. Behavior and motion appear normal in all respects. The species on the left are euglena; the species on the right side is a paramecium. The rate and direction of motion for the paramecia often makes it difficult to capture the organism at this level of magnification. Magnification approx. 600x.

 

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Time lapse images of protozoa that have been subjected to a 0.1% protein solution by weight. These images were captured after a period of exposure to the weak protein solution for approximately 45 – 90 minutes. Euglena are visible in the left photograph (~45 min.) and both paramecium and euglena are visible in the right photograph (~90 min.).The origin and general nature of this particular protein has been described within additional research papers on this site. Behavior and motion do not appear normal. Both species types are significantly impaired in their motion. The vast majority of the euglena appear to be expired at the end of the 90 minute period. The paramecia show a gradual deterioration with very erratic, confused and generally confined motion. Some of the individual paramecium roll into a ball or spherical structure and spin repeatedly until expiring. Magnification approx. 600x.

 

 

This report suggests that the biological and health impacts from this particular protein may be highly significant and detrimental. Additional tests underway support this concern.

 

Clifford E Carnicom
Sep 29 2017

Born Clifford Bruce Stewart
Jan 19 1953

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Mustard Seed Report: Growth Termination

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Mustard Seed Report: Growth Terminated

by

Clifford E Carnicom
Sep 24 2017

The growth of mustard seeds that have been subjected to a specific and isolated protein for one week is now complete This protein is described in greater detail in the paper entitled, Morgellons: Unique Protein Isolated and Characterized (Aug 2017). This protein is derived from the microorganism tentatively identified as a ‘cross-domain bacteria’ (CDB) as described more extensively on this site.

The concentration of the protein solution that was applied to the seeds is 2% by weight. Control solutions with the use of water alone are conducted in parallel for comparison.

The result of this experiment is that germination and growth from the seeds is essentially terminated by the presence of this protein at this concentration level. The control seeds have germinated and flourished normally. Additional trials with a lower concentration of the protein in solution are planned.

Photographs that demonstrate the condition of growth in both cases are shown below:

 

Control_01.jpg Control_02.jpg

Mustard seeds germinated in control water nutrient solution (alone). One week growth period.
Healthy and flourishing growth is evident. Centimeter rule on left photograph; magnification on right photograph approx. 10x.

 

Protein_01.jpg Protein_02.jpg

Mustard seeds subjected to 2% (by weight) protein and water solution. One week growth period.
The termination of the growth process is evident. The early stages of germination can be observed in isolated cases. The vast majority of mustard seeds subjected to the protein solution show no visible germination at the end of the one week period. Centimeter rule on left photograph; magnification on right photograph approx. 10x.

 

This report suggests that the agricultural, biological and health impacts from this particular protein may be highly significant and detrimental. Additional tests underway support this concern.

 

Clifford E Carnicom
Sep 24 2017

Born Clifford Bruce Stewart
Jan 19 1953

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Yeast Deformation: Initial Report

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Yeast Deformation: Initial Report

by

Clifford E Carnicom
Sep 22 2017

 

A yeast culture that has been subjected to an isolated protein is under study. This protein is described in greater detail in the paper entitled, Morgellons: Unique Protein Isolated and Characterized (Aug 2017). This protein is derived from the microorganism tentatively identified as a ‘cross-domain bacteria’ (CDB) as described more extensively on this site.

The purpose of the project is to explore the impact of the protein upon more rudimentary life forms; in this case, a fungus. The protein concentration solution applied to the yeast culture is 0.5% by weight. Control solutions with the use of water and sucrose alone are conducted in parallel for comparison.

The result of this experiment, at this early stage, is that a cellular deformation or alteration of significant proportion has taken place. This suggests that the early growth of this particular fungus is modified in a significant fashion with the inclusion of this protein in the nutrient medium. The act of mutation must be considered as a distinct possibility in this case.

The change occurs primarily upon a surface layer that forms within the culture; this same layer does not develop within the control culture of water and sucrose alone. The act of change is a division process that appears to frequently “join” cells into doublets or triplets, as opposed to a full bud spherical division as expected.

 

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Control growth yeast cells in sucrose and water solution. 72 hour growth period. Cells are generally circular in shape and symmetric. Normal budding and division reproduction process. The appearance of the culture is normal and stable. Magnification approx. 5000x.

 

protein_01.jpg protein_02.jpg
Yeast culture subjected to water, sucrose, and specific protein solution. The isolation of the protein is described further within the research of this site. Concentration of the protein is 0.5% by weight. 72 hour growth period. Unusual growth alterations are evident. Doublet and triplet cell formation appears to be common within the population. Magnification approx. 5000x.

 

The growth process of the yeast culture will continue to be monitored.

Clifford E Carnicom
Sep 22 2017

Born Clifford Bruce Stewart
Jan 19 1953

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Mustard Seed Germination: Initial Report

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Mustard Seed Germination: Initial Report

by

Clifford E Carnicom
Sep 20 2017

 

A series of biological experiments and trials that involve the application of an isolated protein to various growth processes has commenced. This protein is described in greater detail in the paper entitled, Morgellons: Unique Protein Isolated and Characterized (Aug 2017). This protein is derived from the microorganism tentatively identified as a ‘cross-domain bacteria’ (CDB) as described more extensively on this site.

The purpose of the current trial is to explore the impact of the protein upon various plant germinations. A series of germinations is underway; the current report is limited to the advanced germination of mustard seeds within a 48 hour period. The protein solution applied to the seeds is 2% concentration by weight. Control solutions with the use of water alone are conducted in parallel for comparison.

The result of this experiment, at this early stage, is that germination of the seeds is delayed or impeded by the application of the protein solution. This suggests that the early growth of this particular plant is negatively impacted with the inclusion of this protein as a (potential) nutrient source.

The overwhelming majority of the mustard seeds subjected to the protein have not germinated during this brief time period. An optimistic selection of seeds that have been subjected to the protein are shown below; they demonstrate that sprouting to some degree is possible during this same 48 hour period.

The vast majority of the control seeds (i.e., water alone) have germinated normally and they appear to be healthy at this point.

 

Mustard Seed 48 HRS-0002.jpg Mustard Seed 48 HRS-0001.jpg

Mustard seeds germinated in control water nutrient solution (alone). 48 hour germination period.
Germination appears to be normal at this stage.
Magnification approx. 20x

.

Mustard Seed 48 HRS-0003.jpg Mustard Seed 48 HRS-0004.jpg

Mustard seeds germinated in 2% (by weight) protein solution. 48 hour germination period.
The delay and stunting of the germination process is evident. The vast majority of mustard seeds subjected to the protein solution show no visible germination at the end of the 48 hour period. Variation in the surface texture of the seeds in comparison to that of the controls is also apparent.  Magnification approx. 20x.

 

The growth process of this seed trial, along with that of other seed types, will continue to be monitored.

 

Clifford E Carnicom
Sep 20 2017

Born Clifford Bruce Stewart
Jan 19 1953

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A Point of Reckoning : Part II

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A Point of Reckoning:
Part II

by

Clifford E Carnicom
Sep 13 2017

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The organic signature of various proteins that have been isolated from differing sample types and environments has been established to a high level of similarity. The various protein samples have been isolated from:

  1. An identified microorganism (tentatively designated as a cross-domain bacteria, CDB) that has been studied extensively and that is associated with the “Morgellons” condition.
  2. A High Efficiency Particulate Arrestance (HEPA) air filter.
  3. A concentrated rainfall sample.

 

The laboratory methods of analysis include that of:

  1. Organic extraction methods
  2. Liquid column (low pressure) chromatography
  3. Ultraviolet spectroscopy
  4. Visible light spectroscopy (colorimetric test)
  5. Bradford test for protein
  6. Infrared Analysis

 

Additional relevant papers on these and related samples also appear on this site within the research library.

 

Protein Comparison CDB HEPA Rain Aug 16 2017 - 01.JPEG

Infrared analysis and comparison of proteins isolated from a microorganism (CDB) culture, HEPA air filter and rainfall concentrate sample. The concentrations of the samples and the methods and complexity of preparation and protein isolation are vastly different in all cases; nevertheless, a high degree of similarity is apparent with specific functional group signature features. This is especially the case within the ‘functional group’ window within the spectra. The presence of the thiocyanate/isothiocyanate functional group in all samples is an additional highly significant and distinctive feature posing important health considerations.

 

Rainfall Condensed Sample Protein against Control Aug 21 2017.jpg LC HEPA Separation Bradford Verfication Aug 20 2017.jpg
An example of visible light spectral analysis of the Bradford colorimetric test for proteins applied to the rainfall concentrate sample. The Bradford reagent test and VIS-IR spectral analyses have been applied to all sample types identified within this report. Bradford colorimetric test for protein within rainfall concentrate sample.

 

Clifford E Carnicom
Sep 13 2017

Born Clifford Bruce Stewart
Jan 19 1953

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Environmental Filament Project : Metals Testing Laboratory Report

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Environmental Filament, Project:
Metals Testing Laboratory Report

by

Clifford E Carnicorn
Aug 21 2017

A unique form of “environmental filament” material has long been under study at Carnicom Institute. Those familiar with the work here know that the early history of study involves a refusal by the U.S. Environmental Protection Agency to examine that material, and those events are well documented on this site. Many readers are also familiar with the biological components that have accompanied this sample type and the similar refusal by any authoritative agencies to acknowledge the realities of these environmental and health dangers to the public.

This paper will present the data from a high level analytical chemistry examination of this same sample type for metals content. The method of examination is that of inductively coupled plasma mass spectrometry (ICP MS) The testing procedures conform to requirements at the detection level of parts per billion (ppb, or mg/kg). The original observation of the sample is airborne. A low power microscopic image of a second collected sample (identical in nature to that analyzed in the laboratory) follows immediately below:

 

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The test results show the clear presence of numerous metals, frequently to excess levels:

Aluminum
Barium
Calcium
Chromium
Copper
Iron
Lead
Magnesium
Manganese
Nickel
Potassium
Titanium
Vanadium
Zinc

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Clifford E Carnicom
Aug 21 2017

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A Point of Reckoning : Part I

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A Point of Reckoning:
Part I

by
Clifford E Carnicom
Aug 19 2017
Edited Aug 21 2017
Edited Aug 25 2017

Note: Carnicom Institute is not offering any medical advice or diagnosis with the presentation of this information. CI is acting solely as an independent research entity that is providing the results of extended observation and analysis of unusual biological conditions that are evident.  Each individual must work with their own health professional to establish any appropriate course of action and any health related comments in this paper are solely for informational purposes.

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A general equivalency between the organic nature of materials collected with the use of HEPA (High Efficiency Particulate Arrestance) air filters (indoor and outdoor) and a series of concentrated rain samples has been established. This conclusion is based upon the use of infrared analysis, microscopic examination, and visual examination of the materials. The inherent similarity between the historically designated “environmental filament” and the filaments known to be clearly associated with the so-called “Morgellons” condition must also be accepted as a part of this analysis.

This more recent work has been conducted over a period of roughly two years with careful repetitions and redundancies. Fundamentally, the conclusion is logical but nevertheless sweeping in impact; what is in the air is in the water. Furthermore, what is in the air and the water has an important relationship to marked changes in health that affect the general public. What is in the air and in the water is in our bodies.  This state has developed in a global and ubiquitous sense for more than two decades, and we must now all share some responsibility to acknowledge and proclaim our condition on the planet.

The details of the methods will only be briefly summarized here; they involve long term sample collection and a variety of laboratory analyses over extended time. The photographs below will demonstrate the essence of comparison.

The similarity of the infrared plots reveals to us that the basic organic structure of the extracted materials from the air filters and the rain samples are the same. The details of molecular structure inherent within the plot will be reserved for future discussion; the signature aspect of infrared spectroscopy is sufficient at this point to advance the argument.

In addition, microscopic examination reinforces that the air and rainfall biological filament samples are identical. There is little doubt that this biological equivalency is also at the root of the infrared analysis of organics mentioned above.

Additional notes on some of the details of sample types and preparation follow at the end of this report.

C:\Users\Clifford\Documents\Carnicom Institute\A Point of Reckoning\Part I\HEPA-0006.jpg C:\Users\Clifford\Documents\Carnicom Institute\A Point of Reckoning\Part I\HEPA-0023.jpg

Representative “environmental” filaments collected on indoor HEPA Air filter
(blue to left, red to right).
Analysis of the filaments demonstrates properties that are common with filaments
that have been collected from the concentrated rain sample. These filaments are
also representative of those that are associated with the “Morgellons” condition.
The background mesh network (white filaments) is the HEPA air filter itself.
Magnification Approx 150x.

Indoor – Outdoor HEPA Air Filter Comparisons:
Representative “environmental” filaments also collected on an outdoor air HEPA filter
under forced air.  These filaments were collected within a 24 hour exposure  to a new
filter element.  Results are identical between indoor and outdoor exposures.
Magnification Approx 150x.

C:\Users\Clifford\Documents\Carnicom Institute\A Point of Reckoning\Part I\Rainfall Concentrate Analysis 1500x Aug 15 2017_4.jpg

Magnification Approx. 1500x

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Magnification Approx. 5000x.

Filaments collected from rainwater concentrate sample.
Analysis demonstrates properties that are common with filaments collected in the HEPA air filter.
The filaments also demonstrate these same properties that are
associated with the “Morgellons” condition.

 

Observed skin that exhibits symptoms
characteristic of the Morgellons condition.
Filament sample recorded (one of several)
within a portion of the skin condition shown
to the left. Magnification approx. 150x.

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Infrared Plot of HEPA Air Filter Extract

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Infrared Plot of Rainwater Concentrate Sample

Infrared plots to compare the organic nature of the
HEPA air filter extract against the organic nature of the rainfall concentrate.
The samples contain organic materials that are fundamentally of the same nature.

Additional notes:

HEPA Filter(s):

HEPA filters are air filters that are quite effective at trapping materials to the micron size level. They have an interesting history and origin, as they were developed as a part of the Manhattan Project in the 1940’s to trap radioactive materials. This filter type is now in common use and affordable. There is a fair amount of usage of HEPA filters in the history of Carnicom Institute (CI) research, as they are a very effective means of collecting air particulates.

They are also used in commercial aircraft. One of the ironies of the aerosol investigations over the last two decades is that a ready source of sample collection material has always existed; the difficulty is that of access to the samples. CI has long advocated that designing any single aircraft test for sampling the atmosphere is an inefficient, deficient, unnecessary and expensive approach to acquire information about the state of pollution in the atmosphere. This singular test approach has been advocated fruitlessly by several parties over the course of time. The situation is that a massive collection of particulate samples already exists for examination and analysis, but that access to it is not forthcoming. On a hearsay basis, there is information to indicate that the disposal of the filters is carefully controlled (potentially designated as radioactive?).

It is also of interest to mention that, at a very early point of the research, I was given anonymous access in confidence to such a filter from a commercial airliner, along with a laboratory test of the filter for certain metallic elements. That individual remains unknown but he remains deserving of thanks from all of us. To my knowledge, there is no similar test by a member of the public since that time, apparently due to the access issues mentioned above.

That particular filter did show unusual levels of barium in the test results (and calcium to my recollection), and it was one of the harbingers of testing for atmospheric metals that was to follow. At the time of receipt, no laboratory facilities of any kind were available to CI and the physics of the aerosol operations were unknown to the level acquired during subsequent research over the years. Credit is also overdue to AC Griffith, now deceased, for his early role in stimulating interest in the electromagnetic aspects of the aerosol issue. The interplay between ionizable materials and electromagnetics subsequently became a dominant theme of CI research, and the contributions of both of these individuals are to be recognized in that history.

In the case of the current research, two indoor and one outdoor HEPA filters have been examined.  The indoor filters were exposed to long term collection (6-12 months) and the outdoor filter is exposed under short term forced air conditions. Laboratory testing depends upon the sensitivity of the instruments employed and with that sensitivity comes cost. One of the methods of compensating for decreased sensitivity is to allow for an increased time of collection. This is the preference here. As such, one indoor filter was allowed to run its course for approximately 6 months, and the second indoor filter ran close to one full year. All filters are operated approximately 20 feet above ground level. The history of work includes the use of additional outdoor HEPA filters.

Some of the larger pollutants, e.g., the filament samples, can appear quite readily subject to the microscope. The longer term goal in this project was to collect the micron size material that is invisible to the eye until sufficient mass has been collected. This is the source for chemical and spectroscopic testing in this case.

Sample preparation for instrument use is one of the greatest demands in the laboratory environment. It consumes far more effort and time than most people recognize, other than by those involved in the field. In the case of infrared (IR) spectrometry, water is the bane of the testing process and is generally to be avoided in all respects. The HEPA particulates in this case have been dissolved into ethanol, which is a suitable solvent for the preliminary overview that is covered here. The evaporation of the solvent on a suitable substrate will allow the formation of a film which is well suited to infrared spectroscopy. The IR spectra acquired serves two primary purposes:

  1. It serves as a unique fingerprint of the compound(s) in solution
  2. It serves as a useful tool for introductory examination of the molecular structure of the compound(s) in solution

In the case of this paper, the emphasis is only upon the signature aspect of the spectra, as the purpose here is to compare to sampling from a different environment, namely, that of rainfall. This comparison is what is shown above and the point of equality or high similarity is made in the process.

Rainfall Sample:

Rainfall presents even greater difficulties in the sample prep arena. The sensitivity issue discussed above is front and center, and the solution to the problem in this case is to acquire a greater volume of rainfall. Adequate sample volume is definitely an issue, and fortuitous periods of rain will be required.  Non-detrimental evaporation and condensing of the sample will require a fair amount of patience, but it can be accomplished. The samples of this paper were collected in 2016 and were condensed to roughly 5% of their original volume.The organic materials were then removed from the water using a non-polar solvent extraction method for subsequent infrared analysis.  Additional extensive studies were completed on these samples in the previous year, and they have been recorded in a series of papers on this site.

Clifford E Carnicom
Aug 19 2017
Edited Aug 21 2017
Edited Aug 25 2017

Born Clifford Bruce Stewart
Jan 19 1953

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