Tertiary Rainwater Analysis : Questions of Toxicity

Tertiary Rainwater Analysis : Questions of Toxicity

 Clifford E Carnicom
Nov 08 2015

ABSTRACT

This paper presents evidence of a chemical signature that exists within an analyzed rain sample that is characteristic of known toxins and pesticides. The method of analysis used is that of mid-infrared spectroscopy. Specifically, certain functional groups involving sulfur, nitrogen, phosphorus, oxygen, and halogens have been identified in the analysis. It is recommended that the investigation be duplicated by independent researchers to determine if an environmental hazard does exist. If these results are verified to be positive, the source of the contaminants is to be identified and eliminated from the environment.

residual_ir4Infrared Spectrum of Concentrated Rain Water Sample
(Aqueous Influence Removed)

The original rainwater sample volume for this analysis is approximately 3.25 liters.  The sample was evaporated under mild heat to approximately 0.5% of the original volume, or about 15 milliliters.  The sample has previously been shown to contain both aluminum, biological components, and a residue that appears to be an insoluble metallic or organometallic complex.  The target of this particular study is that of soluble organics.

The organic infrared signal within the solution is weak and difficult to detect with the means available; it is further complicated by being present in aqueous solution.  The aqueous influence was minimized by making an evaporated film layer on a KCl cell; the transmission mode was used. The signal is identifiable and repeatable under numerous passes in comparison to the reference background.

The primary conclusion from the infrared analysis is that a core group of elements exists within the solution; these appear to include carbon, hydrogen, nitrogen, sulfur, phosphorus, oxygen and a halogen.  The organic footprint appears to be weak but detectable and dominated by the above heteratoms.

As further evidence for the basis of this report, qualitative tests for an amine (nitrogen and hydrogen), sulfates and phosphates (sulfur, oxygen and phosphorus) have each produced a positive test result.  A qualitative test for a halogen in the concentrated rainwater sample has also produced a positive result; the most likely candidate at this point is the chloride ion.  All elements present have therefore been proven to exist at detectable levels by two independent methods.

This grouping of elements is distinctive; they essentially comprise the core elements of many important, powerful and highly toxic pesticides.   For example, three sources directly state the importance of the group above as the very base of most pesticides:

 

“In pesticides, the most common elements are carbon, hydrogen, oxygen, nitrogen, phosphorus, sulfur and chlorine”.

Pesticide Residues in Food and Drinking Water : Human Exposure and Risks, Dennis Hamilton, 2004.

 

“We can further reduce the list by considering those used most frequently in pesticides: carbon, hydrogen, oxygen, nitrogen, phosphorus, chlorine, and sulfur”.

Fundamentals of Pesticides, A Self-Instruction Guide, George Ware , 1982.

 

“Heteratoms like fluorine, chlorine, bromine, nitrogen, sulfur and phosphorus, which are important elements in pesticide residue analysis, are of major interest”.

Analysis of Pesticides in Ground and Surface Water II : Latest Developments, Edited by H.J. Stan, 1995.

 

It is also true that phosphate diesters are at the core of DNA structure and that many genetic engineering procedures involve the splitting of the phosphate diester complex.

The information provided above is sufficient to justify and invoke further investigation into the matter.  The sample size, although it was derived from an extensive storm over several days in the northwest U.S., is nevertheless limited and quite finite after reduction of the sample volume.  The residual insoluble components (apparently metallic in nature) are also limited in amount and more materials will be required for further analysis.  The signal is weak and difficult to isolate from the background reference; concentration level estimates for elements or compounds (other than that of aluminum which has been assessed earlier) is another entire endeavor.  Systematic, wide-area, and long term testing will be required to validate or refute the results.  All caveats above aside, it would seem that the duty to address even the prospect of the existence of such toxins in the general rainfall befalls each of us.  It would seem wise that this process begins without delay.

There are a few additional comments on this finding that need be mentioned.

The first of these is the issue of local and regional vs. a national and international scope of consideration.  It is understood that pesticides or compounds similar in nature are a fact of our environment, and that considerable awareness and effort is in place to mitigate their damage over decades of use.  Organic farming and genetically engineered crops are two very divergent approaches to reconciliation with the impact of environmental harm, and they are shaping our society and food supply in the most important ways manageable.  Given that the pesticide industry exists, regardless of our varying opinions of merit or harm, I think that it is fair to say that we generally presume that pesticides are under some form of local control.  Our general understanding is that pesticides are applied at ground or close to ground level and are intended to be applied to a specific location or, at most, a region within a defined time interval.

The prospect, even I daresay, the hint, of pesticide or pesticide-like compounds in rainfall is more than daunting.  It seems immediately necessary to consider what scale of operation would support such toxins finding their way into the expanses of atmosphere and rainfall?  For the sake of the general welfare, I think we should all actively wish and seek to disprove the findings within this report.  I will not hesitate to amend this report if honest, fair and accurate testing bears out negative reports over an adequate time period, and my motive never includes sensationalizing an issue.  This is one test, one time, one place, with limited means and support in the process.  I cannot disprove the results at this time and I have an obligation to report on that which seems to be case, uncomfortable as it might be.  It is not the first time that I have been in this situation, and judging from the changes in the the health of the planet that have taken place, it is unlikely to be the last.  The sooner that the state of truth is reached, the better we shall all be for it in any sense that is real.

The second comment relates to the decline of the bee population.  Bees are an indicator species, the canary in the mine, as it were.  The bees and the amphibians have both been ringing their alarm for some time now, and we best not remain passive about finding the reasons for decline.  A minimum of 1/3 of our agricultural economy, and that means food, is dependent upon the bee population for its very existence.  This is no trifling matter, and we all need to get up to speed quickly on the importance of this issue, myself included.

Suffice it to say that compounds of this nature, i.e, historical pesticides like organophosphates and the purported safer and more recent alternatives (e.g., the neonicotinoids), have a very close relationship to the ongoing and often ambiguous studies regarding bee Colony Collapse Disorder (CCD).  From my perspective, it would seem prudent to eliminate the findings of this report as a contributing cause to the problem as promptly as possible.  If that can not be done so readily, then we may have a bigger problem on our hands than is imagined.

One of the interesting side notes is that the elements and groups identified as candidates for investigation actually seem to overlap between the neonicotinioids and the organophosphates.  This includes the nitrogen groups that characterize the neonicotinoids and the phosphate esters that characterize the organophosphates.  If such a combination were at hand, this would seem especially troublesome as both forms remain mired in controversy, let alone any combination thereof.

The third and final comment relates to the toxicity of these compound types in general.  It is not just an issue about bees or salamanders.  These particular compounds have a history and effects that are not difficult for us to research, and we should become aware of their impacts upon the planet quickly enough.  Many of us already are.  The fact is that organophosphates have their origins as nerve gas agents in the pre-World War II era, and in theory their use has been reduced but hardly eliminated.  Residential use is apparently no longer permissible in the United States, but commercial usage still is.  This raises questions on what real effect any such “restrictive” legislation has had.

The neonicotinoids are promoted as a generally safer alternative to the organophosphates, but they are hardly without controversy as well.  They too have strong associations with CCD in the research that is ongoing.  They also are neuro-active insecticides.

It would seem to me that we all have a job to do in getting up to speed on the source, distribution and levels of exposures to insecticide and insecticide related compounds.  A greater awareness of toxins in our environment, in general, also seems in order.  If our general environment has been affected to a degree that has avoided confrontation  thus far, then we need to face the music as quickly as possible.  I trust that we understand the benefits of both rationality and aggressiveness when serious issues face us, and this may be another such time.  I hope that I will be able to dismiss this report in due time; at this time, I cannot.

Sincerely,

Clifford E Carnicom
Nov 05, 2015

Born Clifford Bruce Stewart
Jan 19, 1953

 

Additional Notes:

The preliminary functional group assignments being made to the absorption peaks at this time are as follows (cm-1):

~ 3322 : Amine, Alkynes (R2NH considered)
~ 2921 : CH2 (methylene)
~ 2854 : CH2 (methylene)
~1739 : Ester (RCOOR, 6 ring considered)
~1447 : Sulfate (S=O considered)
~1149 : Phosphate (Phosphate ester, organophosphate considered)
~1072 : Phosphine, amine, ester, thiocarbonyl
~677  : Alkenes, aklynes, amine, alkyl halide

The assignments will be revised or refined as circumstances and sample collections permit, however, as a group they appear to provide a distinctive organic signature.  A structural model may be developed at a future date.

Some chemical compounds which may share some similar properties to that under consideration here include, for example, (not all elements included in any listed compound; only for reference comparison purposes):

p-chlorophenyl (3-phenoxypropyl)carbamate
N-(1-naphthylsulfonyl)-L-phenylalanyl chloride
2,2,2-trichloroethyl 2-(2-benzothiazolyl)dithio-alpha-isopropenyl-4-oxo-3-phenylacetamido-1-azetidineacetate
cytidine monophosphate
diiodobis(triphenylphosphine)nickel(II)

per :
SDBSWeb : http://sdbs.db.aist.go.jp (National Institute of Advanced Industrial Science and Technology, Nov 06 2015)

Secondary Rainwater Analysis : Organics & Inorganics

Secondary Rainwater Analysis :
Organics & Inorganics

Clifford E Carnicom
Nov 04 2015

ABSTRACT

A second rainwater sample has been evaluated. On this occasion, both organic and inorganic attributes of the sample have been examined.  Although the sample investigated is of much larger volume, the results demonstrate an essentially equivalent level of aluminum present to that defined within the earlier report, i.e., approximately 2 PPM.  This magnitude exceeds the US Environmental Protection Agency recommended standards for aluminum in drinking factor by roughly a factor of 10. 

In addition, various organic attributes of the sample are introduced within this report.

rainwater_still_web

 Concentrated Rain Sample under Study in this Report
Distilled Water Reference on Left, Concentrated Rainfall to Right

concentrate
Residual Solid Materials from the Rainwater Sample of this Study

The volume of the sample collected is approximately 6.5 liters over a three day heavy storm period, collected in clean containers that are were exposed to open sky.  The sample was concentrated by evaporation under modest heat to approximately 6% of the original volume.  It is apparent from visual inspection and by visible light spectrometry that the concentrated rainfall sample is not transparent and that it does contain materials to some degree.

Visible Light Spectrum Rainfall2

Visible light spectrum of the concentrated rainfall sample.  The increase in absorption in the lower ranges of visible light correspond to the yellow and yellow-green colors that are observed with the sample.
The pH of the concentrated sample is recorded at 8.5; this value is surprisingly alkaline and indicates the presence of substantial hydroxide ions in solution.  The pH of the solution prior to concentration measures at 7.5; this also must be registered as highly alkaline under the circumstances.

The pH of  ‘natural’ rain water has been discussed in earlier papers and its relationship to the expected value of 5.7 due to the presence of carbonic acid in the atmosphere (carbon dioxide and water).  The departure of natural rainwater from the theoretical neutrality of 7.0 is one aspect of the pH studies that I conducted in conjunction with numerous citizens across the nation some years ago, and these reports remain available.  The current finding is remarkably alkaline and, by itself, is indicative of fundamental acid-base change in the chemistry of the atmosphere.

From those early reports, it may be wise to recall the words of Paul Crutzen, Nobel Prize winner for Chemistry (Atmosphere, Climate and Change, 1995), who stated that the most important chemical attribute of precipitation is indeed the pH value.  It behooves us, as a species, to act rather quickly on any reasonable claim to a significant change in fundamental atmospheric chemistry that may exist.  It must be acknowledged that these same claims now prevail over decades of time, and that any dismissal as an aberration of no consequence is unjustifiably diminutive.

The sample has been examined again for the existence of trace metals using the method of differential cyclic chronopotentiometry, as described in the earlier report. The results are essentially identical to that of the earlier report, and once again the signature of a soluble form of aluminum is detected . The sample in this case, however, is of much larger volume, was collected over a longer duration, and was more highly concentrated that that in the preliminary report.

The concentration level was again determined, and the analysis indicates a level of soluble aluminum within the rainwater sample at 2.0 PPM.  This compares quite closely with the earlier sample result of approximately 2.4 PPM . This determination once again takes into account the concentration process that has been applied to the sample for testing sensitivity purposes.

Two facts bear repeating here:

First, this value exceeds the US Environmental Protection Agency (EPA) standards for drinking water by roughly a factor of 10, again using the most conservative approach possible that can be taken.

Second, the previously referenced U.S. Geological Survey statement from the year of 1967 is valuable both in relation to evaluating the EPA standards as well as assessing the expectations of aluminum concentrations in natural waters:

usgs_quote_1967-2

There is now a necessity to include an additional aspect of the rainfall analysis that has made its presence known more clearly.  This is the case of biologicals.  It is a fact, that in addition to the repeated detection of a trace metal at questionable levels, certain organic constituents are coming to the fore.   The test results are repeatable at this point and these organics will eventually require an equal accounting for their existence.  I will not enter into an extended discussion of their potential significance at this time, as the first and necessary step is to place on the table that which must be confronted.  My introductory suggestion at this point is to become aware of a previous paper on this site, entitled “A New Biology” to gain some familiarity with the scope of the issue . It is fair to say that along with changes of chemistry in this planet, we must also confront certain changes in biology that are in place.  The history of this planet, the cosmos, life and our own species is dynamic, and intelligence itself is partially expressed in the ability to adapt to changing circumstances.  We are in the process, whether we like it or not, of learning if and how quickly we can adapt to changes that have and are taking place, induced or otherwise.  We may also choose whether to participate in the process (hopefully for the betterment of the world, as opposed to its detriment), or if we shall remain ignorant in an effort to ensconce ourselves in a purported comfort zone.

The methods of examination to be presented here are twofold: that of microscopy and that of infrared spectroscopy.  Here are some some images that relate to the fact of the matter; they are repeated in both samples that have been examined:

rain1rain2
rain3rain4

Low Power (~200x) of Biological Filaments Contained in
Residual Materials from Concentrated Rainwater Samples
(The colors of the filaments are a unique characteristic (commonly red and blue) and they exist as an aid to identification with low power microscopy)

rain5rain6
rain7rain8

High Power (~5000x) of Biological Filaments Contained in
Residual Materials from Concentrated Rainwater Samples

These images will not be elaborated on in detail at this time, as it may require a period of time to examine the information that has come forth here.  They most certainly indicate a biological nature that shares a common origin with many of the research topics that have evolved on this site over the years.  It may be worthwhile to begin by becoming familiar with the ‘environmental filament’ issue that is so thoroughly examined on this site.  Since it seems clear that we are indeed dealing with an ‘environmental contaminant’ of sorts, the history of communication with the U.S. Environmental Protection Agency may also be worthy of review.

It would also seem to be the case that a significant portion of the residual material is inorganic as well, as in an insoluble metallic form.  It may be that the insoluble residual material may be composed in part as an organometallic complex, based upon historical findings.

Regardless of the source or impact of these materials, it does seem to fair to state that an accounting for their existence in the atmosphere and rainfall is deserved.  Each of us may wish to play a part in seeking the answers to such issues and questions before us all.  I wish for this to happen, as I suspect many of us know that it is the right thing to do.

 

Clifford E Carnicom
November 01, 2015.

Born Clifford Bruce Stewart
January 19, 1953.

Preliminary Rainwater Analysis : Aluminum Concentration

Preliminary Rainwater Analysis :
Aluminum Concentration


Clifford E Carnicom
Nov 02 2015

ABSTRACT

A method and means to identify the species and concentration of several different trace metals in ionic form has been established.  The method employed is that of differential cyclic chronopotentiometry, which is a subset of the science of voltammetry.  The brief paper presents a preliminary examination of a rainwater sample for the existence of trace metals.  The sample under examination shows the existence of aluminum in a soluble form.  An estimate of the concentration level of the aluminum has been made; this level exceeds that of the recommended standards for drinking water.  The results indicate that public concerns about the toxicity levels of certain trace metals in the general environment are warranted, and that a more thorough evaluation of the state of atmospheric quality by the responsible agencies is required.

rainwater_analysis
Rainwater Sample of this Study Collected under “Clean” Conditions
Note that Visible Pollution is also Evident

The determination of  trace metals can be an expensive and sophisticated proposition.  One of the more modern methods of detection at trace levels involves the use of Inductively Coupled Plasma (ICP); such means and skill sets are not practiced by the public under normal circumstances.  The determination of inorganic compounds at trace levels has always presented a serious challenge to this Institute, and in the past all such efforts have been relegated to that which can be gleaned primarily from qualitative testing methods.  One interesting alternative, with a long history and of increasing importance, is the science of voltammetry.  Many are familiar with the fact that elements and compounds have unique electromagnetic spectrums, such as those employed in the disciplines of spectroscopy including, for example, infrared spectrometry and atomic absorption.  It is valuable to know that many of these same elements also have an ‘electrochemical signature’, and that they behave in unique and identifiable ways when exposed to variations in voltage and current.  It is from this fact that voltammetry was born, and its origin dates back to the the days of Michael Faraday.  The basic principle of voltammetry is to examine the relationships of oxidation and reduction within a medium or a reaction; there are numerous variations upon the specifics of this theme.  Voltammetry equipment is dramatically more modest in cost than ICP and mass spectrometry, and yet it can still produce usable results that are, on many occasions, commensurate with the more advanced equipment and technology.  Such equipment, in is most basic form, is now employed at the Institute and it is yielding promising results in the important domain of inorganic analysis, such as metals and halogens.

The study here refers only to an inorganic analysis that has been made; at a later date a presentation on biological aspects of the rainwater sample will occur as time and circumstances permit.

The rain sample was collected on Oct 30 2015 with new and clean containers with a clear path to the sky above.  The sample was then evaporated to 33% of the original volume for the purpose of increasing the concentration level sufficient for testing purposes.  The sample was compared to a control volume of distilled water.

The potentiostat used in the voltammetry work is a CV-27 model from Bioanalytical Sciences. The unit has passed all test procedures as described in the manual. The output from the potentiostat is coupled to a Pico 2000 series digital oscilloscope, whereby both voltage input and output can be displayed as a function of time. The basic mode of operation for the testing process is therefore one of chronopotentiometry.

A series of calibration tests were made with a variety of trace metals, including calcium, magnesium, sodium, potassium, iron and aluminum.

The goals of the investigations include both the ability to identify the species as well as concentration; both goals have been achieved with the above elements in an ionic state in sufficient concentration, i.e., on the order of a few parts per million (PPM).  The work will extend to other species and combinations thereof in the future.

The particular variation of chronopotentiometry that has been utilized is that of cyclic chronopotentiometry, i.e, the alternating sweep between positive and negative voltages in the effort to identify the peak potential that characterizes the redox reaction of the particular element.

In addition, it has been found that the derivative of the chronopotentiogram is a key and critical factor in the determination of the species.  A careful analysis of the derivative of the cyclic chronopotentiogram can be used with favor to identify the peak potential of the element.

When this point is identified and collated with the identifying element, concentration levels can also be established if a set of known standards is available. Concentration determinations on the order of a few parts per million have been achieved on multiple occasions.

Further careful evaluation of the derivative of the cyclic chronopotentiogram in combination with variable voltage sweeps can be used to identify separate components within a mixture of ionic species; this has been accomplished with a combination of three elements in ionic form in aqueous media to date.

The current work, under these preliminary conditions and examinations, leads to an assessment of a concentration level estimate of aluminum (+3, ionic state) within the rain sample at approximately 2.5 PPM.  A conservative approach in all manners of examination has been adopted in the preparation of this estimate, and the condensing of the sample is accounted for.

The Environmental Protection Agency in 2012 lists the secondary regulations for aluminum in drinking water as being within the range of 0.05 to 0.2 mg/L.  This corresponds to a range of 0.05 to 0.2 PPM for this same standard.  It is an interesting observation within the same report that Secondary Drinking Water Regulations exist as non-enforceable federal guidelines. The wisdom of that classification process can be determined by the reader.

Continuing with the most conservative approach possible, one is led to the assessment that this particular rain sample from a rural location in northern Idaho exceeds the EPA drinking water standard and health advisory by roughly a factor of 12.

The following reference statement from the United States Geological Survey (Bulletin 1827-A, 1967) may be of interest in the evaluation of importance that is to take place:

usgs_quote_1967

It is a point of interest that many individuals have ascribed the detection of aluminum within the atmosphere over a period of many years to my name.  Such was never the case.  My earlier work did indeed establish the precept that ionizable metallic salts are at the core of atmospheric pollution that we now live under, but the testing of aluminum, specifically, was not a part of that process.  The chemistry of aluminum is quite different from that of the alkali earth metals, and the documentation of its existence by others has always raised intriguing questions of physics. Prior to this current work, most of the inorganic analyses that I have made have been restricted to qualitative tests.  No means of testing aluminum at the trace levels has existed for the Institute prior to this occasion.  Hopefully, this situation is now mildly improved with the current voltammetric studies.  This paper adds itself to a long list of documented actions by the citizenry on the consideration that aluminum is certainly, and has been, entitled to.

As a starting point, we might wish to consider the role that aluminum may play within a geoengineered environment, and it may be worthwhile to look at the exothermic energetics of nano-particulates of aluminum under exposure to moisture.  It raises some tantalizing prospects for additional capabilities of an induced or artificial plasma state.

It is also an observation that visible pollutants in rainwater may be most pronounced with the advent of a storm. This is logical, and this has certainly been observed in the cases of excessive fires in this region.  Time will tell if it is the circumstance of other samples.  It remains to be seen how the gradation of pollutants varies with respect to the duration of the rainfall.  Nevertheless, this study does exist as a valid data point and the merit of consideration is not weakened by any progression of dilution.  The concentration gradient with respect to storm length for invisible pollutants, such as those in ionic form, remains as a topic of equal interest for the future.

There is, of course, considerable debate on the issue of the sources of contamination within our water supplies on this planet.  I will not engage in that debate in this paper, as the purpose here is to simply provide another data point of reference that may be of service in helping to establish the accountability that is required.  There are arguments by some that wish to frame a state of ‘normalcy’ for us, regardless of the level of contamination that as a species we now infest ourselves with.  Regardless of various machinations that may be in vogue, we may all ask the questions of where standards evolve from, and whether or not we knowingly wish to deny the legacy of health knowledge that has been acquired over decades, if not centuries.  We should also be called upon to use our united common sense and intuition, pray coupled with the best scientific information available, to act as stewards for our future, and to be worthy of such a title.

Clifford E Carnicom
November 01, 2015.

Born Clifford Bruce Stewart
January 19, 1953.

Environmental Filament : Keratin Encasement

Environmental Filament : Keratin Encasement
Clifford E Carnicom
Jan 07 2013

 

It can now be established with a high degree of certainty that the external casing of the environmental filament samples are composed of keratin or a keratin-like material.  This supposition has been in place for a number of years by this researcher; it can now be demonstrated to be the case by direct chemical and spectroscopic means.  Certain ramifications of this finding, in conjunction with earlier work, are as follows:

1.  It is deduced that the environmental filament is not a naturally occurring material. 

2. The filaments contains non-keratin based chemical and biological components within the internals of the filaments.   Considerable information regarding the nature of the environmental filaments is available on this site; this information has been accrued over a period of several years of progressive research.

3. The emphasis upon study of the filaments is to be directed to the sub-micron components (biological and chemical) that are internal to the filaments.  The keratin aspect of structure is to be interpreted as an encasing mechanism only.

4.  The filaments are not hair or spider webs. 

5.  A false laboratory report has been issued in the past regarding the identification of this filament material (to be discussed in a separate report). 

The primary method by which this conclusion has been reached is with chemical and spectroscopic comparison of a known source of keratin with the environmental filament by similiar means.  This comparison has been made possible with the recent advance in methods of chemical decomposition of keratin based substances by this researcher.  Please see the report entitled “Environmental Filament Penetrated” for this discussion and presentation. 

human hair serbian sample
Human Hair Environmental Filament Sample

Spectroscopic comparisons of keratin obtained from human hair and the same substance obtained from the environmental filament casing are shown immediately below; it will be seen that they are essentially identical.  Additional notes and discussion will follow below the spectra.

spectroscopic analysis of keratin

A visual light spectroscopic analysis of keratin obtained from the decomposition of human hair in combination with ninhydrin and heating.  Human hair is composed predominantly of keratin.

 

spectroscopic analysis of the environmental filament

 

A visual light spectroscopic analysis of the environmental filament after chemical decomposition and in combination with ninhydrin and heat.  It will be seen that the spectrum obtained is essentially identical to the keratin spectrum above. The keratin of the environmental filament is interpreted as an encasement structure and it does not account for the biological components that have been repeatedly identified within this protein housing.

 

colored solutions

 

A photograph of the colored solutions subjected to visual light spectroscopic analysis.  The solutions result from chemical decomposition of keratin based structures, in combination with heat and ninyhdrin.  The solution on the left is derived from the environmental filament sample; the solution on the right is derived from human hair.  Both hues and spectra can vary to some degree by concentration levels within a solution; these examples and spectra indicate a coincidence of relative concentration in each case.

 

 

Additional Notes:

 

Keratin is an especially impervious protein structure.  Observation and study of your own hair is a very good analogy for understanding the hardiness of this particular protein.  During the recent trials of study in decomposition, chemical penetration of hair itself represents an excellent example of the challenge of examination of the environmental filaments and their internals.  Numerous trials were conducted using strong solutions of sulfuric acid, sodium hydroxide, nitric acid, salicylic acid, sodium hypochlorite (bleach), ammonium thioglycolate and others.  All essentially met with failure to the degree needed with the time available.  Although some mild success was achieved with a hair sample, the environmental filament sample remained essentially impervious to almost all methods.  The best success of decomposition has eventually come forth with the use of a commercial hair declogger used in plumbing systems.  This solution is primarily a combination of concentrated sodium hydroxide and concentrated potassium hydroxide.  This solutions is highly caustic. The greater success of this method also becomes  dependent upon the use of applied heat over an extended time period. It was with the use of this method that valid comparisons, both chemically and spectroscopically, could be made.  Considerable work remains before us to acquire the detailed biochemical knowledge of the internal nature of the environmental filaments; this work will continue as the proper resources and equipment avail themselves.

 

MORGELLONS AND RECENT FINDINGS

MORGELLONS  AND RECENT FINDINGS:

PART I : MORGELLONS : A REVERSAL STRATEGY

PART II : PROTEINACEOUS FORM IDENTIFIED

PART III : DIMORPHISM, SYMBIOSIS OR DESIGN

PART IV: MAGNETIC PROPERTIES OF THE GROWTH FORMS

PART V : DNA EXTRACTION

PART VI: THE SERBIAN SAMPLE

PART VII : COLUMN CHROMATOGRAPY

PART VIII : CONFERENCE VIDEO EDITING PROJECT

PART IX : CULTURE GROWTH RATE IMPROVED

PART X : ELECTROPHORESIS PROCESS BEGINSPART

XI : ANOTHER POSITIVE TEST METHOD FOR IRON (Fe+3) IN THE CULTURE

IN PROGRESS
Estimated Completion Date : Can Not Be Estimated At This Time

Clifford E Carnicom
Jan 2012

Note: I am not offering any medical advice or diagnosis with the presentation of this information. I am acting solely as an independent researcher providing the results of extended observation and analysis of unusual biological conditions that are evident.  Each individual must work with their own health professional to establish any appropriate course of action and any health related comments in this paper are solely for informational purposes and they are from my own perspective.

 

PART I : MORGELLONS : A REVERSAL STRATEGY (Dec. 18, 2011)

A viable and tangible strategy to disrupt the growth process of the Morgellons condition, as it exists within the culture form that has been developed, has been established.  This strategy involves the breakdown of certain chemical bonds within an identified proteinaceous complex in a manner that is not harmful to the human body.  The reduction strategy also includes the release of iron that is held within the proteinacous complex in a chelated form.  This strategy has been established with confidence and a repetition of results.  The current work will be applied next directly to oral human samples.  Much time, energy and resources will be required to further investigate, verify and apply this strategy. The preliminary results and the theories are promising at this stage.

biuret iron

To be continued

protein graph

To be continued

PART II: PROTEINACOUS FORM IDENTIFIED

A note to the staff of the Institute tonight (Dec. 2, 2011); this will give some idea as to some of the work in progress…


The existence of a protein within the culture growths has now been established with confidence tonight. I had to do work to eliminate questions of potential contaminants that might have distorted the results. It is also a process of much patience with chromatography, literally drip by drip over many days for each test that is set up. It has taken about 1 1/2 to 2 months to get to this point.

Existence of a protein is eventually of equal importance as that of the iron work. We now have iron and the protein as two primary and identified constituents. This work will raise more questions that it answers, but we need to live with this for now until future means and equipment and methods work their way in. One more reliable way of putting a stop to this fellow is to truly understand the biochemistry and the life cycle of growth; there is then a better chance of interfering with that cycle in a known manner.

The existence of a protein means there is DNA behind it. As you can imagine, the work has actually just begun if we can get these means. Next questions would be what type of protein, what is the function of the protein(s), sequencing of the proteins, etc. Right along with it would be the isolation of DNA, electrophoresis work, etc.  An infra-red spectrophotometer would be a very useful piece of equipment for us on an ongoing basis – we are having to work very hard to get certain results that would be more apparent with the right equipment.

I may put this comment on the paper to get the process started, otherwise I have so many to write I will never get to any of them at the current rate…

Clifford

 A positive Biuret protein test result

A positive Biuret protein test result using a separation of elute from the chromatography column. The sample material is based upon a culture from oral filaments.  The original extraction from the chromatography column is to the left; the positive Biuret result for the existence of a protein is shown on the right with the purple color.  Successful separation on the column has been achieved using various combinations of solvents in combination with a stationary phase

A positive Biuret test result using whey

A positive Biuret test result using whey (lactoferrin) protein for control purposes.  A positive test results in the purplish color shown above.  The Biuret test depends on a copper complex that forms between the protein (peptide bonds) and copper sulfate and an alkaline solution, such as sodium hydroxide.

PART III: DIMORPHISM, SYMBIOSIS OR DESIGN

The morphology, metabolism and life cycle of the “Morgellons” organism, as defined by this researcher, is increasingly being understood.  There are now three scenarios that can be provided that encompass the majority of the understanding that has been achieved.  

The first of these examines a similarity of form, at least in part, to a dimorphic fungal-like organism.  

The second considers the joint existence of bacterial-like and fungal-like organisms in a symbiotic relationship.  

The last raises the spectre of a genetically created or designed organism.  

Each of these scenarios has certain strengths, weaknesses and probabilities of occurrence.  There can also be a degree of overlap between these alternative interpretations.  This paper will discuss what has been discovered, within these three scenarios,  that helps us to potentially define the nature of this unusual organism.

morphology 1

morphology 2

morphology 3

morphology 4

morphology 5

morphology 6

morphology 7

morphology 8

morphology 9

morphology 10

morphology 11

morphology 12

morphology 13

morphology 12

morphology 13

PART IV: MAGNETIC (ELECTROMAGNETIC) PROPERTIES OF THE GROWTH FORMS:

The magnetic (and consequently, the electromagnetic) properties of the primary Morgellons growth form are now proven in a direct fashion.  The video segments below show the response of both the culture derived form and the oral sample to a strong magnetic field.  These demonstrations will call into consideration each of the papers written on the subject of electromagnetics by this researcher.  One such topic will be the extended research that has been done that reveals the ambient presence of unaccounted Extremely Low Frequency (ELF) energy over a testing period of several years.  The human electromagnetic system operates primarily within the ELF portion of the electromagnetic spectrum.  The sensitivity and response of the Morgellons growth form to the electromagnetic spectrum is another of the many primary fields of research that requires funding, resources and skilled personnel to complete.  The identified presence of iron and ferromagnetic compounds within the growth forms establishes the basis of this future research, along with the direct demonstration of the magnetic response shown below:


To be continued.


PART V: DNA EXTRACTION

dna 1 dna 2 dna 3

To be continued.


PART VI: THE SERBIAN SAMPLE

To be continued.

serbia 1 serbia 2
serbia 3
serbia 4 serbia 5
serbia 6 serbia 7
serbia 8

PART VII: COLUMN CHROMATOGRAPHY

To be continued.

column 1

column 2

 

PART VIII : CONFERENCE VIDEO EDITING PROJECT

 

To be continued.

PART IX : CULTURE GROWTH RATE IMPROVED

To be continued.

X : ELECTROPHORESIS PROCESS BEGINS

ELECTROPHORESIS 1

Starch Gel Electrophoresis Applied to Proteinacous Samples : Initial Tests Underway

ELECTROPHORESIS 2

ELECTROPHORESIS 3

Starch Gel Electrophoresis : Trial Runs of Test Dyes and Blood Sample.   Left photograph shows methylene blue dye migration towards the negative terminal. Arrows on right photograph depict origins of placement.  Blood sample shows both positive and negative charged protein component separation at lower portion of right photograph.  Eosin test case on upper left of right photograph; migration toward positive terminal  Methods remain under development; no successful separation of presumed culture based proteinacous component at this time.

To be continued.

XI :ANOTHER POSITIVE TEST METHOD FOR FERRIC IRON (Fe3+) IN THE CULTURE

Another test method has been developed to detect and establish the presence of iron in the Fe3+ state within the culture growth that is based upon the oral samples.  The test is positive.  The further significance of this test is that it has been applied directly to the proteinaceous complex that has been extracted from the culture with the use of column chromatography.  This further substantiates the case that the proteinaceous complex itself contains iron in the ferric state and that this iron is bound to certain amino acids that are under examination as candidates.   It will be possible to determine the concentration of the iron within the proteinaceous complex through spectrometry.  The test is based upon the use of ammonium thioglycolate.

Clifford E Carnicom
(born Clifford Bruce Stewart Jan 19 1953)

MORGELLONS: FIRST OBSERVATIONS

MORGELLONS:
FIRST OBSERVATIONS
Clifford E Carnicom
Aug 12 2006
Edited Aug 16 2006
Copyright 2006 by Clifford E Carnicom and Jan Smith

This paper is being presented in two stages. The first paper will describe a series of observations under the microscope at relatively high power. The subject of observation is sample material received from an individual that displays the symptoms of what is now known as Morgellons disease. The Morgellons disease is characterized in part by the presence of a host of unusual skin conditions, commonly including persistent lesions and unusual fibers or filaments. The second paper will be commentary on the Morgellons issue from the perspective of this researcher; it will be presented separately at a later date.

The illness causes much pain and suffering. The acceptance of the illness by the formal medical community remains controversial, despite increasing and widespread evidence of its existence. This inquiry is prompted by the finding that despite several years of presumed research on the Morgellons issue, there apparently are no suitably magnified images of the filaments available to the public. Despite recent media attention to the issue, it also appears to be commonly claimed that there are not sufficient resources available to conduct suitable examinations. This presentation will seek to address this problem to the degree possible here.

morgellons observations 1
Original Sample Envelope Postmarked Jul 14 2006.
Samples are from the subject directly and were sent to me upon my request.

Materials were received in mid July after lengthy discussion with the individual. This person has previously made numerous observations and discussions available to the public; photographs were limited to a magnification of 200x by the equipment that was available. I offered to conduct a microscopic study at higher magnification, to photograph the results and to make this information available to the public. This is the primary purpose of this first report; no concerted effort to evaluate the nature of the materials will be made on this page. This page represents several hours of study under the microscope of one portion of one sample, and much more work remains to be done. I may or may not have the means or resources to continue this study and it is questionable that this work should occur under the domain of citizen activism.

One of the goals of this paper is to also provide the reader with a sense of scale, and to show a progression from the original materials as they exist from the body to the highest magnification possible with my equipment. The materials received were packaged carefully, thoroughly labeled and in good order. The subject’s description of the physical symptoms encountered are thorough and complete. Any questions put forth to the individual about the illness have been answered in full candor and detail. The subject has provided numerous samples to a medical doctor in the past but no specific response, descriptions, photos or analysis have apparently been provided in return. Detailed information or replies from any formal medical representative, non-profit support or research groups, educational institutions and government agencies appears to be grossly deficient. There appears to be no adequate response to the individual’s many appeals for analysis of the illness symptoms and physical manifestations. The ramifications of a potentially large scale health issue that may be affecting a much larger portion of the population than is currently recognized must be considered.  In addition to the suffering that has been endured, those leading individuals that have come forth with their appeals for assistance have in the main been derided, denied or refused.  At the very least this group requires our humanitarian compassion and medical assistance.

It is also necessary to confront directly the numerous claims of “delusion” that are commonly circulated in conjunction with the public reports on this issue, and to seek out if there are any agendas that may be associated with this characterization of the illness. It must also be asked why the citizenry is in the position of having to provide the information on this page to the public. The question of unknown health risks to the public (and even to this researcher) should be addressed.

It is a legitimate question to seek out if these materials are of any unusual form or nature, as this does not appear to be properly and publicly addressed at this point. Adequate images under the microscope may be of help here.

morgellons observations 2
One of several original sample materials received in sealed plastic container.
Materials photographed prior to handling and observation.
Materials stated to come from a lesion on the torso.
Embedded filaments within the lesion material are visible
(isolated fibers just visible to the naked eye).
This container measures approximately 1 inch (2.5cm) across.
Observations to follow are from this sample only.

morgellons observations 3
Second photograph of original sample materials received.
Additional samples exist for future analysis.
Several filaments that emanate from the lesion material are visible to the naked eye

Observations to follow are from this sample only.

The fibers that are visible and that emanate from the lesion material in the photographs above are the subject of the photographs below. Five magnification levels are available with the equipment being used: approximately 700x, 1400x, 2800x, 5600x and 8600x.  Digital magnification of the final image can be increased further if the situation warrants it and if the image quality supports the enlargement. The limit of conventional optical magnification is approximately 2000x. The higher levels shown here have been achieved with the combination of digital camera equipment (primarily astronomic) and a decent optical microscope. It is believed that these images are the first available publicly that show internal detail of the fibers that are apparently representative of the Morgellons condition. Much deserves to be explained and accounted for with the disclosures that follow, as it becomes quickly evident that these are not typical nor uniform fibers.

The first image shown below is at a magnification of approximately 700 times. At the level of 700x, there is relatively little detail that can be seen. The photograph is adequate, however, to obtain a first estimate of its width; this first measurement is approximately 10 to 12 microns.  This measurement already seriously calls into question any claim of these fibers being a human hair, as they will measure from approximately 60 to 100 microns in width.  The irregular form of the fiber and twisting that is apparent further eliminates any realistic comparison to a human hair. At this point recall that most images that have been made available have been at a level of 200x or less; this already explains why little information about the appearance of the fibers, let alone any internal structure, is available to the public to review.  I have encountered two extremely high magnification images taken with an electron microscope, however, it will be seen that no internal detail is available from those images. As there is no commentary associated with those images, there can be no further explanation of that deficiency at this point.

morgellons observations 4
Magnification approximately 700x.
Approximate dimension in width : 10-12 microns.
No major distinguishing characteristics visible.
Indications of some internal structure to fiber may be apparent.
Suitable for measurement and comparison to human hair.
Some unevenness in size noted and ability of the fiber to fold or twist is visible..
Two different fibers examined; both appear essentially identical at this point.

The next presentation will be that of two control photographs for purposes of comparison and to show the capability of the modified microscopy equipment that is being used. THESE ARE FOR CONTROL PURPOSES ONLY AND ARE NOT ASSOCIATED WITH THE SAMPLE MATERIALS IN ANY WAY.  The first photograph will be that of a human hair, also at a magnification of approximately 700 times.  The next photograph will be that of a human blood cell at approximately 8600 times; a human blood cell measures on the order of 6-8 microns across.  For further comparison, bacteria are commonly on the order of up to 10 microns in size, and viruses are usually  1 micron or less.  An asbestos fiber is on the order of 2 microns.  For further comparisons and extensive fiber studies, please consult some of the earlier work on this site.

morgellons observations 5
Human Hair : FOR CONTROL PURPOSES ONLY
Magnification approximately 700x..
Note smooth outline and uniform size.
Measurement : approximately 65 microns across.
No significant internal structure or form apparent.

morgellons observations 6
Human Blood Cell : FOR CONTROL PURPOSES ONLY
Magnification approximately 8600x.
Approximate size of cell : 7 microns in diameter
This image represents the upper end of quality
and magnification of the equipment being used in this report.

The next set of photographs, the primary focus of this report, will show a series of photographs at 1400x and 5600x levels of magnification.  At this stage of the research project, I will largely let the photographs speak for themselves, with minor comments to assist in their interpretation.  The next two photographs at 1400x now begin to show some interesting features and form that have not been visible with the initial work.  First, it is observed that the fibers have a much more complicated internal structure than was discernible at low magnification.  In addition, there is more variance in the dimensions of the fiber than is originally evident.  Both of these factors alone begin to seriously question or eliminate claims of commonly known fibers, be they artificial or natural.  In the media reports alone, there are now reports of attempted matching of the fiber form using large forensic databases and a complete failure of identification in that attempt.  One of the objectives of this report is to allow the public itself to see why that failure is likely occurring.

There is a second revelation at this level of magnification and observation.  What appears to be a single filament coming from the lesion material is actually much more complicated and that much exists that is not visible to the naked eye.  In the second of these two photographs, notice the rather complicated web of numerous fibers.  This arrangement was most certainly not visible by eye when this sample was placed under the microscope.  It is at this point that much greater interest is to be attached to the identification of these filaments, as well as whatever structures may be contained within.  We also notice, particularly in the second photograph of greater translucency, that internal, much smaller structures of elliptical form exist.  This begins to strongly suggest a biological nature to the fibers, and a case for ruling out human hairs as well as any common fiber form, natural or artificial, is now made.

At this point, it is at least appropriate to address the rather massive efforts that have been and that are being made to characterize this illness as a psychological problem of the afflicted individuals.  This effort will be addressed more completely in the commentary section that shall follow at a later time.  In the interim, however, if the materials being shown here are representative of the Morgellons condition, such efforts to foist a perception of  “delusion” upon the public can only be interpreted as a ruse of the highest order in an effort to conceal, deny and avoid the true issues that we are facing. The reports of occurrence of this illness are increasing and they are global at this stage.  It is reasonable to inquire as to what agendas may be in place to so forcefully attempt to influence the public perception of this condition or disease.

morgellons observations 7
Magnification approximately 1400x.
Note variation in fiber form and internal structure becoming evident.
Notice irregularities on the surface of the fiber.
Notice translucent quality of the fiber.

morgellons observations 8
Magnification approximately 1400x.
Numerous fibers are now available; this conglomerate not visible to the naked eye.
Notice internal structures becoming increasingly visible.
Biological natures are more strongly indicated at this point of observation.

The next and final set of photographs will be at 5600x.  Several important discoveries take place.  It is now quite common within certain segments of the primary fiber to find an internal sub-fibrous structure. It can now be seen that what appears to be a single fiber is composed of innumerable sub-fibers, and that these sub-fibers measure at the micron or sub-micron level.  There is no known previous disclosure of this fact on the Morgellons condition and a much more complex interpretation of the actual nature of the fibers must now be proposed.  Secondly, internal spherical or elliptical structures now appear within the primary fiber, measuring on the order of 1 micron (virus size).  It is now a compelling priority to identify these structures and their functions, including the internal micron sized sub-fibers.

morgellons observations 9
Magnification approximately 5600x.
Notice internal filament structure within the fiber.
Width of the internal fibrous structure is at the micron or sub-micron level.

morgellons observations 10
Magnification approximately 5600x.
Notice internal generally circular structures.
Strongly indicative of a biological nature at this point.
These structures measure on the order of 1 micron (virus size).
Increasingly complex internal nature of the original sample fiber is now evident.

The last major discovery by observation at this point is what appears to a “budding” structure of some sort.  These structures appear on the edge of the fiber at irregular intervals.  These structures contain two further components within.  The first of these are spherical or elliptical structures at the micron level within an encasing, translucent shell.  In addition, innumerable fibers at the sub-micron level emerge from the budding structure.  The budding structures are highly indicative of a growth or reproductive process, and they may be related to the spread of the disease.

morgellons observations 11
Magnification approximately 5600x.
“Budding” structures are apparent on the sides of the fiber at occasional locations.
The budding structures contain internal structures at the roughly micron or sub-micron size.
Budding structures also often contain innumerable filaments within,
measuring apparently at the sub-micron level (Limit of equipment reached).
Reproduction and growth of the primary fiber structure may be closely linked to these budding structures.
The budding structures generally appear to be quite complex in form, structure and organization.

morgellons observations 12
Magnification approximately 5600x.
Complex internal organization of sub-fibers and structural forms is apparent.

morgellons observations 13
Magnification approximately 5600x.
This photograph shows the ability of the fiber to be folded and/or twisted.
Internal parallel organization of sub-fibers is visible.
Non-uniformity of the fibers  dimensions is also evident.
Transverse separation or structure also visible in lower right of image.

morgellons observations 14
Magnification approximately 5600x.
Additional budding structure visible on the edge of the primary fiber.
Complex internal micron size structures within.
Translucent encasement that is indicative or suggestive of reproductive capability.

morgellons observations 15
Magnification approximately 5600x.
Additional budding structure visible on the edge of the primary fiber.
Complex internal micron size structures within.

The conclusion of this report is necessarily brief at this time.  The basic conclusions that can be made are as follows.  First, there has been a complete failure of the formal medical community, non-profit organizations and government to adequately research and distribute information to the public on the nature of the Morgellons condition.  If the samples studied and shown here are in any way representative of the Morgellons disease, they show that any effort to influence the public to accept this evidence as being of  psychological origin or as insignificant are disingenuous at the highest level.  Any motive of secrecy and or misinformation is to be confronted directly and disclosed.  The so-called efforts at research by various organizations, including non-profit, university and government are to be called into question; there is a serious lack of informing the public as to the basic nature of the condition. No citizen should be assuming the risk of attempting to identify the nature of this illness.  The traditional medical community and government health organizations have already displayed an appalling failure of addressing the urgency of this matter.  I call upon all of those individuals or groups with the proper resources to strike to the core of this issue as quickly as possible, and to disclose all results of the findings to the public as they occur.

Clifford E Carnicom
August 12 2006

Notes:
Additional research and or information from other sources will be linked into this report as it becomes available.

Additional commentary of the general state of findings on the Morgellons issue will be presented at a later date.
The results of this report are of a preliminary nature, and they are restricted to the materials that have been provided by a single individual.