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Protein Separation, Microbe Identification & Blood Transformation

This paper is Part Three of a Five Part series.

There is no possibility of avoiding the following statement, and it will therefore be declared at the onset.

Direct evidence indicates that the application of a low magnitude electrical current can or will transform a blood sample to the point that it is no longer recognizable as blood in any conventional sense, and the nature of the blood is then dominated by the presence of the cross-domain bacteria (CDB) (CI nomenclature) microbial life form and its manifestations.  The blood sample examined in great depth is one of those that has been discussed previously within this specific five part report series.

This work was completed between February and May of 2022 in a field setting with portable instrumentation.  The work was motivated by the rather profound blood coagulation activity recorded in a previous paper, Blood Alterations I : Coagulation (Jul 2022).  The record of observations for this work is contained in Carnicom Institute Laboratory Notebooks, Vol 26 and 27; these volumes have been made available to the public through this site. The work is extensive and detailed in nature and the laboratory notes will serve as the most accurate record of what transpired.  This paper will be a summary of the processes and results that have unfolded in the course of this recent work; the previous paper entitled, Blood Alterations II : Means & Methods is a helpful adjunct to this purpose.

An explanation is now required to justify the opening statement of this paper.  Please be forewarned that a series of complex steps was involved in making this conclusion.

The first stage of examination suggested that AC voltammetry would be a suitable method to explore some of the chemical changes that might be occurring within the blood samples to cause the profound coagulation that appears.  The general benefits and application of AC Voltammetry has been described in the previous paper of the series and will not be repeated here.  The use of this method was entirely exploratory at this point, and there was no expectation whatsoever of what was eventually to occur.

The sample under examination here is the introduction of a couple of drops of fresh blood into a vial of 3-4 ml. of distilled water.  The author is aware of the osmotic influence of distilled water upon erythroctyes (red blood cells) and this poses no limitation to the problem of blood composition at this point.  The specific conditions of the AC voltammetry technique employed are described in detail within the laboratory notebooks that are mentioned in earlier reports.

One of the first observations is that the application of current in this mode to the blood sample produced a “frothy”, or foam-like material that would rise to the top of the solution. As such explorations have never been conducted before, there were no presumptions in place as to what would occur or what might be found.  This mode of electrochemistry was repeated upon the blood sample on numerous occasions, and the frothy material (foam, or “suds” like) occurred in a repeatable fashion each time.

Numerous other observations were recorded in the process.  An additional observation is that over time the vial blood solution underwent a series of active electrochemical reactions and then eventually clarified itself with a settling of bright red material on the bottom of the tube, presumably erythrocytes subjected to osmotic disruption.  Additionally, the activity level of the various electrodes involved was recorded within the notes.

As the process was repeated over many trials, the frothy material at the top of the tube garnered increased interest.  The obvious and foremost action to take is to examine the frothy material under the microscope.  This presents the first phenomenal observation within the electrochemical process; this result was and still is completely unexpected; and for that matter, it remains completely unexplained.  One example of the repeatable result is shown below:

Human Blood Sample Previously Subjected to AC Voltammetry Electrochemistry
CDB Presence & Filament Formation is Evident
Magnification ~ 1500x.

What is seen here is known not to be blood in any conventional or conceivable sense.  This author has an extensive database of study of the cross-domain bacteria (CDB) (CI nomenclature) microbe  that is known to be deeply embedded within human( and animal) blood samples that have been examined over recent decades.  This growth event that has taken place from as a result of applied electrochemical energy is a complete and total match with that microbe, form and size.  It is also important to note that no such significant filament growth (characteristic of the CDB) was apparent in earlier observations of this same blood sample, and as that same sample has been presented in the earlier report.  See Altered Blood I : Coagulation.

An earlier paper that provides a great deal of information on the growth variations in the CDB and associated filament growth structure is entitled CDB : Growth Progressions (Jun 2014).  A couple of images are extracted from that paper and shown below the coincidence and relations that exist between the the CDB and filament formation:


CDB – Linear Alignment Process Prior to
Filament Formation
Original Magnification Approx. 5000x
Source: Growth Progressions, Jun 2014


 Filament Development with Internal CDB
Original Magnification Approx. 5000x
Source: Growth Progressions, Jun 2014

Besides the obvious filament structure formation in the blood sample as a consequence of applied current, we can further open discussion on the “foamy”, or frothy material that is present.  We can ask what is the general occurrence of foam or suds like materials in nature, and similarly what is their expected chemical nature?

There are two frequent sources of foam materials that appear in the natural world, one is the result of pollution and the other is the result of denatured proteins.  Proteins therefore now emerge as a primary target of interest in addition to the presence of the sub-micron CDB and the filament structures.  Indeed, we do observe a granular type formation visible in the slides as it can be seen below:


Granular structure apparent within blood sample, ~1500 x.
Electrical current has previously been applied to the sample.
Primary candidate determination is that of a generated and foreign denatured protein.


The result above is therefore one of our early clues that our foam production in the blood via AC voltammetry is comprised of three major components: the CDB, the filaments, and a suspected foreign CDB generated protein.  Recall that the foam material is a distinct chemical separation from the remaining settled red contents in the vial, and which are to be evaluated later in the process.

The question of whether a protein exists can be answered with the use of a suitable protein detection reagent test, as has been described within the previous Blood Alteration II : Means & Methods paper.  The result of this test is positive, and at this point the protein is suspected to be a foreign protein that is generated as a result of the electrochemical process.  Further analysis of the nature of that protein will occur at a later stage in this research presentation.  An alkaline solution of sodium hydroxide can be used to solubilize the protein.

All three components have also been isolated, identified and analyzed extensively within CDB culture work over past decades.  Our major surprise here, however, is that these components appear to be synthesized in some fashion, in a relatively brief time period,  as a result of electrical current conditions applied to the blood.

Protein determination is enhanced considerably with the additional use of visible light spectrometry in combination with the reagent that has been developed.  Subtle changes in concentration and behavior (e.g, proteins subject to enzymatic action) can be effectively determined with the combined use of a suitable colored reagent and visible light spectrometry. Near infrared spectrometry can also be used as a supportive technique to establish the existence of nitrogen based functional groups.

The trials that employed AC voltammetry were repeated on numerous occasions with identical results.

An important question now arises at this point.  It has been seen thus far that the method of AC voltammetry under specific parameters is sufficient to produce the remarkable changes in blood character; the additional question that now comes to mind is whether or not AC voltammetry is necessary to produce the change.  It has been mentioned previously that AC voltammetry is a more complex technique than some others that might be used within elecrochemistry, although not so much that it would be considered difficult to achieve from a system development/environmental point of view.

But it does beg the question of whether a simpler electrochemical technique might produce this same change in the nature of blood.  The answer is yes.

It has been found that chronopotentiometry is sufficient to produce the same alteration and transformation that includes CDB and filament appearance, as well as the same protein formation.  Chronopotentiometry has been described previously and is a relatively simple technique where a constant DC current is applied to the sample and the voltage (potential) is measured as a function of time.  This is a much simpler “system” to develop and the fact that this method is sufficient to produce the radical blood transformation is an important finding.  AC voltammetry will reenter the picture when protein analysis is discussed in a later paper.

It is appropriate now to mention the magnitudes of various currents that are involved here. In general, the trials underway involve the use of at most a few milliamps (mA) (single digits) of current; this is quite low from any practical perspective.  The instrument under use is quite sensitive and is able to measure current down to the nano and pico amp ranges.

It is also worthwhile to now question the amount of current in the body that is considered to be dangerous, harmful, or even lethal. It requires a surprisingly low current in the body to produce harm or death.  Research indicates that pain will be felt with internal current at approximately 10 mA  or greater, and that death can occur with a current greater than approximately 60 mA.  The highly significant finding here is that current on the order of just a few milliamps is sufficient to produce a complete transformation of the blood character and constitution as it relates to the CDB microbial presence.  It is difficult to conceive of blood as functioning in the body at any level once protein and microbial transformation has taken place.

This set of facts should be held foremost as we confront the findings disclosed here.

Next, we recall that the electrochemical process (chronopotentiometry is sufficient) creates two primary separations, the frothy material under study above, and a bright red solid layer that will eventually settle to the bottom as the solution clarifies.  This lower layer contains the bulk of the mass of the end result.  By most expectations, one might easily surmise that this bottom layer would be primarily or entirely comprised of blood cells, even if osmotically disrupted.  There is expected to be at least some hemoglobin contribution to this layer, however, there are also obvious foreign biologicals that predominate here as well.  Selected microphotographs will again reveal the seriousness of the problem.  The images are not anomalous, but are representative of the blood sample that has been transformed with the application of low level electrical current.


CDB Filament Formation in Blood Sample (Lower Layer)
Previously Subjected to Low Level Electrical Current


Linear, or “Field” Alignment of Presumed CDB Generated Protein
in Blood Previously Subjected to Low Level Electrical Current


CDB Filament Formation in Blood Sample (Lower Layer)
Previously Subjected to Low Level Electrical Current


Linear, or “Field” Alignment of Presumed CDB Generated Protein
in Blood Previously Subjected to Low Level Electrical Current


Linear, or “Field” Alignment of Presumed CDB Generated Protein
in Blood Previously Subjected to Low Level Electrical Current


Presumed CDB Generated Protein
Combined with Presumed Osmotically Disrupted Erythrocytes
in Blood Previously Subjected to Low Level Electrical Current.
Occasional Erythrocyte is Visible.


CDB Filament Pair Formation in Blood Sample (Lower Layer)
Previously Subjected to Low Level Electrical Current

The above images are remarkable; it would be difficult to dispute this.  They clearly demonstrate the most profound transformation of blood that one might even consider to be possible.  The transformation cannot be considered anomalous at this point; it is, to the contrary, representative of the sample.  The CBD microbial presence and its various manifestations now dominant the nature of the blood sample.  This transformation results from the application of low magnitude electrical current to the blood sample. This same blood sample is known to contain the CDB microbial form prior to application of the electrochemical energy.  Thus far, no blood samples examined are immune from the presence of the CDB, except in variation by degree of presence.

The “alignment” phenomena of the generated proteins also opens up an entire field of research and discovery that is completely unexplored at this point.  Magnetics and electromagnetic field influence, natural or otherwise, are undoubtedly lead candidates for investigation.  There will also be, without doubt, no exceptions provided for the influence of “vaccines” or other injections upon the human condition.

It will go almost without saying that this portion of the sample also tests positive for the existence of protein, however the degree of separation between hemoglobin and a foreign generated protein is not determinable with the reagent test alone.  Analysis of the protein constitution presented within a later paper will shed some additional light on this uncertainty.

The information above provides the basis and justification for the opening statement of this paper.  Please distribute and preserve this report series globally.  Thank you.

Clifford E Carnicom
Jul 31 2022

Born Clifford Bruce Stewart
Jan 19 1953



Blood Alterations IV : Protein Analysis

Blood Alterations V : Implications & Consequences



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